IFI-S · Glycan Peak

Specimen SS-F-SWT-079 // SWEET · IFI-S

Glycan Peak V-IV-A

Capsicum chinense

Pepper

Tier VGlycan Peak

Target Trait

4.5×

fold-change

N-glycoprotein sweetness signal transduction amplification

Technical Specifications

Genomic Edits

4

GC Content

48.5%

Construct Length

6,740 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

450

Pathway Edits

  • TAS1R2 N-glycosylation site Asn-44 remodeling for glycan shielding
  • UGT76G1 steviol glycoside biosynthesis pathway upregulation cassette
  • PLCβ2 signaling cascade efficiency optimization via PDZ domain edit
  • TAS1R3 cysteine-rich domain glycosylation pattern restructuring

Key Interventions

Glycan shield engineering at VFT domain dimer interfaceReceptor dimerization stability enhancement via disulfide lock insertionIP3-mediated Ca2+ release amplification in taste receptor cellsN-acetylglucosamine branching pattern optimization at Asn-44

IFI Subscale Profile

IFI Profile

IFI-S
450
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

plant-based sweetness optimization for reduced-sugar product lines

License

$499 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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