IFI-S · Glycan Peak

Specimen SS-F-SWT-072 // SWEET · IFI-S

Glycan Peak IV-IV-B

Capsicum chinense

Pepper

Tier IVGlycan Peak

Target Trait

4.4×

fold-change

N-glycoprotein sweetness signal transduction amplification

Technical Specifications

Genomic Edits

5

GC Content

48.0%

Construct Length

6,400 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

440

Pathway Edits

  • TAS1R2 N-glycosylation site Asn-44 remodeling for glycan shielding
  • UGT76G1 steviol glycoside biosynthesis pathway upregulation cassette
  • PLCβ2 signaling cascade efficiency optimization via PDZ domain edit
  • TAS1R3 cysteine-rich domain glycosylation pattern restructuring
  • TRPM5 gating kinetics enhancement via N-terminal glycan modulation

Key Interventions

Glycan shield engineering at VFT domain dimer interfaceReceptor dimerization stability enhancement via disulfide lock insertionIP3-mediated Ca2+ release amplification in taste receptor cellsN-acetylglucosamine branching pattern optimization at Asn-44Steviol glycoside aglycone docking pocket remodeling in TAS1R2 VFT

IFI Subscale Profile

IFI Profile

IFI-S
440
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance high-intensity sweetener potency multiplier for industrial applications with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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