IFI-S · Saccharide Scale

Specimen SS-F-SWT-038 // SWEET · IFI-S

Saccharide Scale V-PR-B

Capsicum annuum

Pepper

Tier VSaccharide Scale

Target Trait

3.45×

fold-change

TAS1R2/TAS1R3 sucrose binding affinity enhancement

Technical Specifications

Genomic Edits

4

GC Content

46.7%

Construct Length

6,740 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R2/TAS1R3

IFI Score

345

Pathway Edits

  • TAS1R2 Venus flytrap domain loop-3 mutagenesis (Y103F, E302K)
  • TAS1R3 cysteine-rich domain disulfide bridge optimization
  • Gα gustducin coupling interface stabilization via helix-8 edit
  • cAMP second-messenger amplification cassette integration

Key Interventions

VFT binding pocket residue substitution to increase sucrose EC50 shiftTMD allosteric potentiator binding site expansion via S3-S4 loop editcAMP-mediated secondary messenger cascade amplificationReceptor surface expression enhancement via ER export signal optimization

IFI Subscale Profile

IFI Profile

IFI-S
345
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance functional sweetness enhancement for dietary applications with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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