IFI-S · Saccharide Scale

Specimen SS-F-SWT-032 // SWEET · IFI-S

Saccharide Scale IV-IV-B

Capsicum annuum

Pepper

Tier IVSaccharide Scale

Target Trait

2.93×

fold-change

TAS1R2/TAS1R3 sucrose binding affinity enhancement

Technical Specifications

Genomic Edits

4

GC Content

45.5%

Construct Length

6,240 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

293

Pathway Edits

  • TAS1R2 Venus flytrap domain loop-3 mutagenesis (Y103F, E302K)
  • TAS1R3 cysteine-rich domain disulfide bridge optimization
  • Gα gustducin coupling interface stabilization via helix-8 edit
  • cAMP second-messenger amplification cassette integration

Key Interventions

VFT binding pocket residue substitution to increase sucrose EC50 shiftTMD allosteric potentiator binding site expansion via S3-S4 loop editcAMP-mediated secondary messenger cascade amplificationReceptor surface expression enhancement via ER export signal optimization

IFI Subscale Profile

IFI Profile

IFI-S
293
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance sugar reduction without flavor compromise in baked goods with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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