IFI-S · Saccharide Scale

Specimen SS-F-SWT-025 // SWEET · IFI-S

Saccharide Scale IV-AG-A

Capsicum annuum

Pepper

Tier IVSaccharide Scale

Target Trait

2.55×

fold-change

TAS1R2/TAS1R3 sucrose binding affinity enhancement

Technical Specifications

Genomic Edits

3

GC Content

44.8%

Construct Length

6,080 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R2/TAS1R3

IFI Score

255

Pathway Edits

  • TAS1R2 Venus flytrap domain loop-3 mutagenesis (Y103F, E302K)
  • TAS1R3 cysteine-rich domain disulfide bridge optimization
  • Gα gustducin coupling interface stabilization via helix-8 edit

Key Interventions

VFT binding pocket residue substitution to increase sucrose EC50 shiftTMD allosteric potentiator binding site expansion via S3-S4 loop editcAMP-mediated secondary messenger cascade amplification

IFI Subscale Profile

IFI Profile

IFI-S
255
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

sugar reduction without flavor compromise in baked goods

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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