IFI-S · Saccharide Scale

Specimen SS-F-SWT-022 // SWEET · IFI-S

Saccharide Scale III-PR-B

Capsicum annuum

Pepper

Tier IIISaccharide Scale

Target Trait

2.42×

fold-change

TAS1R2/TAS1R3 sucrose binding affinity enhancement

Technical Specifications

Genomic Edits

3

GC Content

44.3%

Construct Length

5,580 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R2/TAS1R3

IFI Score

242

Pathway Edits

  • TAS1R2 Venus flytrap domain loop-3 mutagenesis (Y103F, E302K)
  • TAS1R3 cysteine-rich domain disulfide bridge optimization
  • Gα gustducin coupling interface stabilization via helix-8 edit

Key Interventions

VFT binding pocket residue substitution to increase sucrose EC50 shiftTMD allosteric potentiator binding site expansion via S3-S4 loop editcAMP-mediated secondary messenger cascade amplification

IFI Subscale Profile

IFI Profile

IFI-S
242
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance low-calorie confectionery ingredient development with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$199 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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