IFI-S · Saccharide Scale

Specimen SS-F-SWT-011 // SWEET · IFI-S

Saccharide Scale II-BL-A

Capsicum annuum

Pepper

Tier IISaccharide Scale

Target Trait

1.65×

fold-change

TAS1R2/TAS1R3 sucrose binding affinity enhancement

Technical Specifications

Genomic Edits

2

GC Content

42.4%

Construct Length

4,920 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS1R2/TAS1R3

IFI Score

165

Pathway Edits

  • TAS1R2 Venus flytrap domain loop-3 mutagenesis (Y103F, E302K)
  • TAS1R3 cysteine-rich domain disulfide bridge optimization

Key Interventions

VFT binding pocket residue substitution to increase sucrose EC50 shiftTMD allosteric potentiator binding site expansion via S3-S4 loop edit

IFI Subscale Profile

IFI Profile

IFI-S
165
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

natural sweetener augmentation in beverages

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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