IFI-S · Saccharide Scale

Specimen SS-F-SWT-007 // SWEET · IFI-S

Saccharide Scale I-IV-A

Capsicum annuum

Pepper

Tier ISaccharide Scale

Target Trait

1.2×

fold-change

TAS1R2/TAS1R3 sucrose binding affinity enhancement

Technical Specifications

Genomic Edits

1

GC Content

41.2%

Construct Length

4,260 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

120

Pathway Edits

  • TAS1R2 Venus flytrap domain loop-3 mutagenesis (Y103F, E302K)

Key Interventions

VFT binding pocket residue substitution to increase sucrose EC50 shift

IFI Subscale Profile

IFI Profile

IFI-S
120
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

reduced-sugar food formulation platform

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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