Kokumi Peak V-AG-A

Allium sativum

Garlic

Tier VKokumi Peak

Target Trait

6×

fold-change

Peak kokumi intensity fold-change at sensory panel assessment

Technical Specifications

Genomic Edits

GC Content

59.2%

Construct Length

3,800 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

900

Pathway Edits

Maximum kokumi peak formulation
Complete allosteric network redesign
Cooperative binding from Hill≥4 engineering
Sub-millisecond signal onset optimization
Explosive Ca2+ transient design
Full ER Ca2+ store mobilization circuit
Plasma membrane channel storm coordination
Mitochondrial Ca2+ buffer saturation design
CaMKII Thr286 permanent autophosphorylation analog
CREB1 transcriptional amplifier lock
Immediate-early gene cascade entrainment
Epigenetic H3K27ac mark maintenance for sustained expression

Key Interventions

maximum-intensity kokumi peak circuitexplosive Ca2+ transient with full ER mobilization and sustained transcriptional lock

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

900

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

World-class kokumi pinnacle ingredient for flagship product differentiation

License

$499 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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