IFI-K · Kokumi Peak

Specimen SS-F-KOK-192 // KOKUMI · IFI-K

Kokumi Peak IV-IV-B

Allium sativum

Garlic

Tier IVKokumi Peak

Target Trait

6.04×

fold-change

Peak kokumi intensity fold-change at sensory panel assessment

Technical Specifications

Genomic Edits

12

GC Content

57.7%

Construct Length

3,480 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

906

Pathway Edits

  • Ultra-high kokumi peak engineering
  • CaSR positive cooperativity Hill≥3 design
  • Signal threshold ultrasensitivity switch
  • Zero-delay Ca2+ release circuit
  • Parallel IP3/RYR2 cascade firing
  • SERCA complete inactivation at peak
  • NCX reverse-mode activation
  • TRPC3/6 sustained influx channel insertion
  • Calcineurin-independent NFAT bypass
  • Ryanodine receptor sensitization cluster edit
  • PLCδ supplementary amplifier insertion

Key Interventions

ultrasensitive switch behavior for sharp kokumi peakparallel Ca2+ release pathway co-firing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
906
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Elite koji fermentation flavor intensifier

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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