IFI-K · Kokumi Peak

Specimen SS-F-KOK-183 // KOKUMI · IFI-K

Kokumi Peak III-IV-A

Allium sativum

Garlic

Tier IIIKokumi Peak

Target Trait

4.5×

fold-change

Peak kokumi intensity fold-change at sensory panel assessment

Technical Specifications

Genomic Edits

10

GC Content

56.2%

Construct Length

3,160 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

675

Pathway Edits

  • Kokumi peak timing optimization circuit
  • Ca2+ oscillation frequency tuning
  • CAMK2B autophosphorylation lock
  • CaM-CaMKK axis strengthening
  • AMPK-mTOR cross-talk removal
  • Eukaryotic initiation factor 4E sensitization
  • Polysome loading for key effector proteins
  • Translational control circuit optimization
  • PKC isozyme selection edit for optimal signal character

Key Interventions

Ca2+ oscillation frequency and amplitude co-optimizationCAMK2 autophosphorylation lock for sustained signal

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
675
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Ultra-premium miso and soy sauce kokumi amplifier

License

$149 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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