IFI-K · Calcium Flux

Specimen SS-F-KOK-080 // KOKUMI · IFI-K

Calcium Flux V-IV-B

Allium cepa

Onion

Tier VCalcium Flux

Target Trait

3.68×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

9

GC Content

50.8%

Construct Length

2,810 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

552

Pathway Edits

  • Full CaSR signaling hub remodel
  • Negative regulator cluster deletion
  • NFAT transcriptional positive feedback
  • CAMK4 nuclear translocation edit
  • RGS protein family silencing
  • IP3K-mediated IP4 production boost
  • PMCA efflux pump downregulation
  • Mitochondrial Ca2+ uptake optimization

Key Interventions

complete Ca2+ signaling network reengineeringmaximal flux for elite kokumi response

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
552
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Bioactive kokumi compound for functional food development

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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