IFI-K · Calcium Flux

Specimen SS-F-KOK-072 // KOKUMI · IFI-K

Calcium Flux IV-IV-B

Allium cepa

Onion

Tier IVCalcium Flux

Target Trait

3.2×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

8

GC Content

49.3%

Construct Length

2,490 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

480

Pathway Edits

  • CaSR homodimer interface strengthening
  • Allosteric modulator binding site engineering
  • Gβγ coupling optimization
  • TRPV4 co-expression cassette
  • IP3R2/3 sensitization dual edit
  • STIM1-Orai1 SOCE potentiation
  • PKA inhibitory cascade removal

Key Interventions

multi-channel Ca2+ influx coordinationSOCE pathway co-activation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
480
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fine dining application with nuanced Ca2+ response

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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