Calcium Flux III-PR-A

Allium cepa

Onion

Tier IIICalcium Flux

Target Trait

2.35×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

GC Content

47.8%

Construct Length

2,170 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

352.5

Pathway Edits

CaSR cysteine-rich domain stabilization
PKC-δ feedback suppression
RYR2 sensitization edit
CAMK2A amplifier cassette
CaM binding site optimization
NCX1 transporter silencing

Key Interventions

sustained Ca2+ oscillation inductionCAMK2A-mediated potentiation loop

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

352.5

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Plant-based meat kokumi profile construction

License

$149 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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