IFI-K · Calcium Flux

Specimen SS-F-KOK-058 // KOKUMI · IFI-K

Calcium Flux III-AG-B

Allium cepa

Onion

Tier IIICalcium Flux

Target Trait

2.7×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

7

GC Content

47.8%

Construct Length

2,170 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

405

Pathway Edits

  • CaSR cysteine-rich domain stabilization
  • PKC-δ feedback suppression
  • RYR2 sensitization edit
  • CAMK2A amplifier cassette
  • CaM binding site optimization
  • NCX1 transporter silencing

Key Interventions

sustained Ca2+ oscillation inductionCAMK2A-mediated potentiation loop

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
405
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Plant-based meat kokumi profile construction

License

$199 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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