IFI-K · Calcium Flux

Specimen SS-F-KOK-055 // KOKUMI · IFI-K

Calcium Flux II-IV-A

Allium cepa

Onion

Tier IICalcium Flux

Target Trait

1.93×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

5

GC Content

46.3%

Construct Length

1,850 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

289.5

Pathway Edits

  • CaSR Venus flytrap domain Ser170→Ala
  • Gαq11 gain-of-function edit
  • SERCA pump partial silencing
  • ER Ca2+ store loading edit
  • PLCβ scaffold optimization

Key Interventions

CaSR allosteric sensitivity enhancementER Ca2+ reserve amplification

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
289.5
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Dairy analog mouthfulness enhancement platform

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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