Calcium Flux II-PR-B

Allium cepa

Onion

Tier IICalcium Flux

Target Trait

2.22×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

GC Content

46.3%

Construct Length

1,850 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

333

Pathway Edits

CaSR Venus flytrap domain Ser170→Ala
Gαq11 gain-of-function edit
SERCA pump partial silencing
ER Ca2+ store loading edit
PLCβ scaffold optimization

Key Interventions

CaSR allosteric sensitivity enhancementER Ca2+ reserve amplification

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

333

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Dairy analog mouthfulness enhancement platform

License

$149 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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