IFI-K · Calcium Flux

Specimen SS-F-KOK-041 // KOKUMI · IFI-K

Calcium Flux I-AG-A

Allium cepa

Onion

Tier ICalcium Flux

Target Trait

1.5×

fold-change

CaSR-mediated intracellular Ca2+ flux fold-change

Technical Specifications

Genomic Edits

4

GC Content

44.8%

Construct Length

1,530 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

225

Pathway Edits

  • CaSR expression cassette insertion
  • Onion ACS1 promoter swap
  • γ-Glu-Cys synthetase OE
  • IP3R1 copy number increase

Key Interventions

CaSR expression boost via strong promoterγ-Glu peptide substrate pool expansion

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
225
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fermented food kokumi foundation ingredient

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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