IFI-C · Thermal Contrast

Specimen SS-F-CLB-127 // COOL BURN · IFI-C

Thermal Contrast I-IV-A

Capsicum annuum

Pepper

Tier IThermal Contrast

Target Trait

1.6 ratio

TRPM8/TRPV1 ratio

TRPM8/TRPV1 activation ratio

Technical Specifications

Genomic Edits

6

GC Content

44.0%

Construct Length

1,920 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPM8 + TRPV1

IFI Score

640

Pathway Edits

  • TRPV1 sensitization pathway (PKC-ε node) upregulation
  • TRPM8 concurrent activation locus dual-edit
  • Capsaicin biosynthesis branch point KAT2 precision edit
  • Menthol-capsaicin synergy regulatory cassette insertion
  • Channel co-expression construct PGK::TRPM8-T2A-TRPV1
  • Desaturase FAD7 edit for membrane fluidity optimization

Key Interventions

Contrasting thermal signal co-activation engineeringSequential cool-then-burn perception timing controlTRPV1 sensitization for burn-phase amplitude amplificationTRPM8 cool-phase priming for perceptual contrast effectSynergistic channel cross-activation cascadeMembrane lipid remodeling for enhanced channel sensitivity

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
640
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Complex spice-and-cool flavor systems, hot-sauce innovation with cooling finish, fermented food flavor engineering, and multi-sensory culinary gastronomy applications.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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