IFI-B · Taste Rescue

Specimen SS-F-BTR-185 // ZERO-BITTER · IFI-B

Taste Rescue IV-AG-A

Brassica oleracea var. italica

Broccoli

Tier IVTaste Rescue

Target Trait

87.5%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

11

GC Content

48–52%

Construct Length

3,200 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

875

Pathway Edits

  • Full taste rescue construct
  • TAS2R38 non-taster phenotype stabilization
  • Glucosinolate 75% pathway reduction
  • Sweetness enhancement locus
  • Bitterness masking compound synthesis

Key Interventions

Complete phenotype restorationDual pathway optimization platform

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
875
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium personalized broccoli for direct-to-consumer subscription platforms offering taste-profiled produce.

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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