IFI-B · Suppressor Grade

Specimen SS-F-BTR-071 // ZERO-BITTER · IFI-B

Suppressor Grade IV-IV-A

Brassica oleracea var. sabellica

Kale

Tier IVSuppressor Grade

Target Trait

53.75%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

6

GC Content

44–48%

Construct Length

2,700 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

537.5

Pathway Edits

  • TAS2R38 multi-site suppression
  • AOP2/AOP3 ablation
  • CYP79F1 downregulation
  • Gluconapin/Sinigrin simultaneous reduction
  • Brassinin pathway edit

Key Interventions

Multi-glucosinolate suppression platformTAS2R38 complete inactivation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
537.5
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium kale for meal kit, foodservice, and restaurant applications demanding consistent flavor quality at scale.

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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