IFI-B · Suppressor Grade

Specimen SS-F-BTR-063 // ZERO-BITTER · IFI-B

Suppressor Grade III-IV-A

Brassica oleracea var. sabellica

Kale

Tier IIISuppressor Grade

Target Trait

47.5%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

5

GC Content

43.2–47.2%

Construct Length

2,380 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

475

Pathway Edits

  • TAS2R38 gain-of-function suppression
  • AOP2/AOP3 dual targeting
  • Sinigrin reduction
  • Gluconapin pathway edit

Key Interventions

AOP2/AOP3 dual ablationTriple glucosinolate pathway suppression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
475
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial kale for salad blends, ready-to-eat applications, and meal kits requiring broad household acceptance.

License

$149 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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