IFI-B · Suppressor Grade

Specimen SS-F-BTR-056 // ZERO-BITTER · IFI-B

Suppressor Grade II-IV-B

Brassica oleracea var. sabellica

Kale

Tier IISuppressor Grade

Target Trait

47.44%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

5

GC Content

42.4–46.4%

Construct Length

2,280 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

474.4

Pathway Edits

  • TAS2R38 allele modification
  • Sinigrin pathway attenuation
  • AOP2 downregulation

Key Interventions

Dual glucosinolate suppressionAOP2 downregulation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
474.4
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Functional kale chips and snack applications with improved palatability enabling category expansion to general consumers.

License

$149 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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