IFI-B · Suppressor Grade

Specimen SS-F-BTR-047 // ZERO-BITTER · IFI-B

Suppressor Grade I-IV-A

Brassica oleracea var. sabellica

Kale

Tier ISuppressor Grade

Target Trait

35%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

3

GC Content

41.6–45.6%

Construct Length

1,740 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

350

Pathway Edits

  • TAS2R38 coding region edit
  • Sinigrin biosynthesis attenuation

Key Interventions

Sinigrin pathway attenuationTAS2R38 allele switch

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
350
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Smoothie and juice kale with reduced astringency for mainstream consumer adoption beyond the health enthusiast segment.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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