IFI-B · Brassica Clear

Specimen SS-F-BTR-039 // ZERO-BITTER · IFI-B

Brassica Clear V-IV-A

Brassica oleracea var. italica

Broccoli

Tier VBrassica Clear

Target Trait

45%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

5

GC Content

43.6–47.6%

Construct Length

2,840 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

450

Pathway Edits

  • TAS2R38 complete suppression
  • MYB28/MYB29/MYB76 full knockout
  • CYP79F1/CYP79F2 deletion
  • Progoitrin pathway ablation
  • BGLU enzyme activity reduction

Key Interventions

Complete TAS2R38 suppressionFull glucosinolate pathway removalSystemic palatability enhancement

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
450
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Elite palatability-certified broccoli for premium retail, subscription programs, and direct-to-consumer health channels.

License

$499 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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