IFI-B · Brassica Clear

Specimen SS-F-BTR-034 // ZERO-BITTER · IFI-B

Brassica Clear V-AG-B

Brassica oleracea var. italica

Broccoli

Tier VBrassica Clear

Target Trait

51.75%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

6

GC Content

44–48%

Construct Length

3,020 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

517.5

Pathway Edits

  • TAS2R38 complete suppression
  • MYB28/MYB29/MYB76 full knockout
  • CYP79F1/CYP79F2 deletion
  • Progoitrin pathway ablation
  • BGLU enzyme activity reduction

Key Interventions

Complete TAS2R38 suppressionFull glucosinolate pathway removalSystemic palatability enhancement

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
517.5
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Elite palatability-certified broccoli for premium retail, subscription programs, and direct-to-consumer health channels.

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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