IFI-B · Brassica Clear

Specimen SS-F-BTR-031 // ZERO-BITTER · IFI-B

Brassica Clear IV-IV-A

Brassica oleracea var. italica

Broccoli

Tier IVBrassica Clear

Target Trait

38.75%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

4

GC Content

42.8–46.8%

Construct Length

2,520 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

387.5

Pathway Edits

  • TAS2R38 receptor gene silencing
  • MYB28/MYB29 knockout
  • CYP79F1/CYP79F2 downregulation
  • Progoitrin reduction

Key Interventions

TAS2R38 receptor ablationTriple MYB transcription factor knockoutAliphatic glucosinolate reduction

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
387.5
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Specialty broccoli for health-focused CPG products requiring minimal bitterness with retained glucoraphanin nutrition.

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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