IFI-B · Brassica Clear

Specimen SS-F-BTR-023 // ZERO-BITTER · IFI-B

Brassica Clear III-IV-A

Brassica oleracea var. italica

Broccoli

Tier IIIBrassica Clear

Target Trait

32.5%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

4

GC Content

42–46%

Construct Length

2,200 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

325

Pathway Edits

  • TAS2R38 promoter silencing
  • MYB28/MYB29 dual attenuation
  • CYP79F1 downregulation

Key Interventions

TAS2R38 receptor suppressionDual MYB transcription factor knockoutCYP79F1 downregulation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
325
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium broccoli for restaurant and foodservice channels demanding reliable palatability across growing seasons.

License

$149 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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