IFI-B · Brassica Clear

Specimen SS-F-BTR-016 // ZERO-BITTER · IFI-B

Brassica Clear II-IV-B

Brassica oleracea var. italica

Broccoli

Tier IIBrassica Clear

Target Trait

30.19%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

4

GC Content

41.2–45.2%

Construct Length

2,100 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression)

IFI Score

301.9

Pathway Edits

  • TAS2R38 5-prime UTR silencing
  • MYB28 attenuation
  • GST upregulation

Key Interventions

TAS2R38 transcript suppressionMYB28 downregulationProgoitrin decrease

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
301.9
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial broccoli for school nutrition programs and family meal kits requiring consistent reduced bitterness.

License

$149 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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