IFI-B · Brassica Clear

Specimen SS-F-BTR-013 // ZERO-BITTER · IFI-B

Brassica Clear II-PR-A

Brassica oleracea var. italica

Broccoli

Tier IIBrassica Clear

Target Trait

26.25%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

3

GC Content

42.2–46.2%

Construct Length

1,920 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS2R38 (suppression)

IFI Score

262.5

Pathway Edits

  • TAS2R38 5-prime UTR silencing
  • MYB28 attenuation
  • GST upregulation

Key Interventions

TAS2R38 transcript suppressionMYB28 downregulationProgoitrin decrease

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
262.5
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Commercial broccoli for school nutrition programs and family meal kits requiring consistent reduced bitterness.

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Zero-Bitter · IFI-BAll Modalities