IFI-B · Brassica Clear

Specimen SS-F-BTR-002 // ZERO-BITTER · IFI-B

Brassica Clear I-AG-B

Brassica oleracea var. italica

Broccoli

Tier IBrassica Clear

Target Trait

23%

% reduction

% bitter reduction (TAS2R38 suppression)

Technical Specifications

Genomic Edits

3

GC Content

40.8–44.8%

Construct Length

1,740 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

230

Pathway Edits

  • TAS2R38 promoter CpG methylation
  • GST upregulation

Key Interventions

TAS2R38 epigenetic silencingGlucosinolate baseline reduction

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
230
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fresh-market broccoli with improved acceptance among bitter-sensitive consumers and children.

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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