IFI-A · Vinifera Reserve

Specimen SS-F-AST-200 // ASTRINGENCY · IFI-A

Vinifera Reserve V-IV-B

Vitis vinifera

Grape

Tier VVinifera Reserve

Target Trait

2.3 g/L

mg/L

Ultra-high condensed tannin and galloylation index

Technical Specifications

Genomic Edits

17

GC Content

49.5%

Construct Length

8,240 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

1,150

Pathway Edits

  • LAR ultra-expression cassette with 3'UTR stabilization
  • ANR multi-locus integration with synthetic promoter
  • DFR/F3H/F3'5'H triple-gene operon construct
  • TT12/TT19 dual-transporter co-overexpression
  • MYB5/MYB9/TT2 transcription factor cascade
  • Galloylquinic acid pathway activation via HCT/AT2
  • Flavonoid glycosyltransferase suppression for aglycone enrichment
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Ultra-high proanthocyanidin galloylation index maximizationProcyanidin oligomer distribution engineering toward DP6+Condensed tannin–anthocyanin copigmentation complex formationVacuolar membrane transport capacity saturationChalcone flux bottleneck elimination at CHS/CHI nodeCell wall–bound tannin fraction optimizationNADPH regeneration pathway enhancement for sustained polyphenol fluxShikimate pathway carbon redirection for phenylpropanoid precursorsSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
1,150
IFI-F
—

Use Case

Ultra-premium wine tannin standardization, barrel-aging analog functional ingredients, reserve-grade polyphenol nutraceuticals, high-galloylation food antioxidant production, next-generation astringency calibration standards. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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