IFI-A · Proanthocyanidin Tier

Specimen SS-F-AST-150 // ASTRINGENCY · IFI-A

Proanthocyanidin Tier IV-PR-B

Vitis vinifera

Grape

Tier IVProanthocyanidin Tier

Target Trait

1.7 g/L

mg/L

High-molecular-weight proanthocyanidin complex

Technical Specifications

Genomic Edits

14

GC Content

48.7%

Construct Length

7,100 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

862.5

Pathway Edits

  • LAR high-expression cassette (leucoanthocyanidin reductase)
  • ANR multi-copy integration (anthocyanidin reductase)
  • DFR allelic replacement with high-activity variant
  • TT19 glutathione S-transferase upregulation
  • TT12 vacuolar transporter amplification
  • MYB9 transcription factor constitutive expression
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

High-molecular-weight proanthocyanidin chain elongationProcyanidin B-type and A-type ratio optimizationVacuolar sequestration efficiency maximizationProtein-binding tannin surface area engineeringAstringency persistence and mouthcoating modulationSecondary cell wall polyphenol deposition enhancementPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
862.5
IFI-F
—

Use Case

Fine wine flavor engineering, premium spirits tannin modulation, functional grape seed extract production, high-tannin phenolic food additives, advanced mouthfeel design in fermented beverages. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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