Proanthocyanidin Tier IV-AG-B

Vitis vinifera

Grape

Tier IVProanthocyanidin Tier

Target Trait

1.7 g/L

mg/L

High-molecular-weight proanthocyanidin complex

Technical Specifications

Genomic Edits

GC Content

49.3%

Construct Length

7,100 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

862.5

Pathway Edits

LAR high-expression cassette (leucoanthocyanidin reductase)
ANR multi-copy integration (anthocyanidin reductase)
DFR allelic replacement with high-activity variant
TT19 glutathione S-transferase upregulation
TT12 vacuolar transporter amplification
MYB9 transcription factor constitutive expression
T-DNA right-border optimized integration cassette
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

High-molecular-weight proanthocyanidin chain elongationProcyanidin B-type and A-type ratio optimizationVacuolar sequestration efficiency maximizationProtein-binding tannin surface area engineeringAstringency persistence and mouthcoating modulationSecondary cell wall polyphenol deposition enhancementAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

862.5

IFI-F

—

Use Case

Fine wine flavor engineering, premium spirits tannin modulation, functional grape seed extract production, high-tannin phenolic food additives, advanced mouthfeel design in fermented beverages. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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