IFI-A · Proanthocyanidin Tier

Specimen SS-F-AST-135 // ASTRINGENCY · IFI-A

Proanthocyanidin Tier II-IV-A

Vitis vinifera

Grape

Tier IIProanthocyanidin Tier

Target Trait

1.3 g/L

mg/L

High-molecular-weight proanthocyanidin complex

Technical Specifications

Genomic Edits

10

GC Content

47.4%

Construct Length

5,360 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

650

Pathway Edits

  • LAR high-expression cassette (leucoanthocyanidin reductase)
  • ANR multi-copy integration (anthocyanidin reductase)
  • DFR allelic replacement with high-activity variant
  • TT19 glutathione S-transferase upregulation
  • TT12 vacuolar transporter amplification
  • MYB9 transcription factor constitutive expression
  • Cell-free wheat germ extract reconstitution system

Key Interventions

High-molecular-weight proanthocyanidin chain elongationProcyanidin B-type and A-type ratio optimizationVacuolar sequestration efficiency maximizationProtein-binding tannin surface area engineeringAstringency persistence and mouthcoating modulationSecondary cell wall polyphenol deposition enhancementSP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
650
IFI-F
—

Use Case

Fine wine flavor engineering, premium spirits tannin modulation, functional grape seed extract production, high-tannin phenolic food additives, advanced mouthfeel design in fermented beverages.

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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