IFI-A · Proanthocyanidin Tier

Specimen SS-F-AST-121 // ASTRINGENCY · IFI-A

Proanthocyanidin Tier I-AG-A

Vitis vinifera

Grape

Tier IProanthocyanidin Tier

Target Trait

1.2 g/L

mg/L

High-molecular-weight proanthocyanidin complex

Technical Specifications

Genomic Edits

9

GC Content

49.3%

Construct Length

4,860 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

600

Pathway Edits

  • LAR high-expression cassette (leucoanthocyanidin reductase)
  • ANR multi-copy integration (anthocyanidin reductase)
  • DFR allelic replacement with high-activity variant
  • TT19 glutathione S-transferase upregulation
  • TT12 vacuolar transporter amplification
  • MYB9 transcription factor constitutive expression
  • T-DNA right-border optimized integration cassette

Key Interventions

High-molecular-weight proanthocyanidin chain elongationProcyanidin B-type and A-type ratio optimizationVacuolar sequestration efficiency maximizationProtein-binding tannin surface area engineeringAstringency persistence and mouthcoating modulationSecondary cell wall polyphenol deposition enhancementAgrobacterium co-cultivation with acetosyringone induction

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
600
IFI-F
—

Use Case

Fine wine flavor engineering, premium spirits tannin modulation, functional grape seed extract production, high-tannin phenolic food additives, advanced mouthfeel design in fermented beverages.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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