IFI-A · Phenol Peak

Specimen SS-F-AST-104 // ASTRINGENCY · IFI-A

Phenol Peak III-IV-B

Camellia sinensis

Tea

Tier IIIPhenol Peak

Target Trait

1.1 g/L

mg/L

Catechin/epicatechin monomeric pool

Technical Specifications

Genomic Edits

11

GC Content

45.6%

Construct Length

5,960 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

575

Pathway Edits

  • CHS overexpression (chalcone synthase)
  • CHI amplification (chalcone isomerase)
  • ANS upregulation (anthocyanidin synthase)
  • ANR high-flux variant (anthocyanidin reductase)
  • F3'H enhancement (flavonoid 3'-hydroxylase)
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Catechin biosynthesis flux maximizationEpicatechin gallate esterification pathway activationEGCG precursor pool expansionFlavan-3-ol branch competition reductionMalonyl-CoA supply amplification via ACC overexpressionSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
575
IFI-F
—

Use Case

Premium green tea analogs, EGCG-enriched functional beverages, high-astringency specialty teas, antioxidant-forward RTD beverages, nutraceutical catechin extract production. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$199 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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