IFI-A · Phenol Peak

Specimen SS-F-AST-082 // ASTRINGENCY · IFI-A

Phenol Peak I-AG-B

Camellia sinensis

Tea

Tier IPhenol Peak

Target Trait

920 mg/L

mg/L

Catechin/epicatechin monomeric pool

Technical Specifications

Genomic Edits

8

GC Content

47.1%

Construct Length

4,640 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

460

Pathway Edits

  • CHS overexpression (chalcone synthase)
  • CHI amplification (chalcone isomerase)
  • ANS upregulation (anthocyanidin synthase)
  • ANR high-flux variant (anthocyanidin reductase)
  • F3'H enhancement (flavonoid 3'-hydroxylase)
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Catechin biosynthesis flux maximizationEpicatechin gallate esterification pathway activationEGCG precursor pool expansionFlavan-3-ol branch competition reductionMalonyl-CoA supply amplification via ACC overexpressionAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
460
IFI-F
—

Use Case

Premium green tea analogs, EGCG-enriched functional beverages, high-astringency specialty teas, antioxidant-forward RTD beverages, nutraceutical catechin extract production. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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