IFI-A · Polyphenol Scale

Specimen SS-F-AST-050 // ASTRINGENCY · IFI-A

Polyphenol Scale II-AG-B

Sorghum bicolor

Sorghum

Tier IIPolyphenol Scale

Target Trait

575 mg/L

mg/L

Proanthocyanidin polymer chain length

Technical Specifications

Genomic Edits

7

GC Content

45.2%

Construct Length

4,500 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

287.5

Pathway Edits

  • ANR overexpression (anthocyanidin reductase)
  • F3H upregulation (flavanone 3-hydroxylase)
  • DFR amplification (dihydroflavonol 4-reductase)
  • TT12 vacuolar transporter enhancement
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Condensed tannin polymerization degree controlEpicatechin unit flux maximizationPolyphenol oxidase suppression for stabilityProanthocyanidin B-type linkage engineeringAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
287.5
IFI-F
—

Use Case

Craft cider and beer astringency enhancement, sorghum-based tannin additives, specialty food ingredient production, nutraceutical polyphenol concentrates. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$149 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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