IFI-A · Polyphenol Scale

Specimen SS-F-AST-048 // ASTRINGENCY · IFI-A

Polyphenol Scale I-IV-B

Sorghum bicolor

Sorghum

Tier IPolyphenol Scale

Target Trait

460 mg/L

mg/L

Proanthocyanidin polymer chain length

Technical Specifications

Genomic Edits

6

GC Content

43.3%

Construct Length

4,000 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

230

Pathway Edits

  • ANR overexpression (anthocyanidin reductase)
  • F3H upregulation (flavanone 3-hydroxylase)
  • DFR amplification (dihydroflavonol 4-reductase)
  • TT12 vacuolar transporter enhancement
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Condensed tannin polymerization degree controlEpicatechin unit flux maximizationPolyphenol oxidase suppression for stabilityProanthocyanidin B-type linkage engineeringSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
230
IFI-F
—

Use Case

Craft cider and beer astringency enhancement, sorghum-based tannin additives, specialty food ingredient production, nutraceutical polyphenol concentrates. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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