IFI-A · Tannin Grade

Specimen SS-F-AST-034 // ASTRINGENCY · IFI-A

Tannin Grade V-AG-B

Vitis vinifera

Grape

Tier VTannin Grade

Target Trait

460 mg/L

mg/L

Condensed tannin accumulation

Technical Specifications

Genomic Edits

9

GC Content

42.3%

Construct Length

5,680 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

230

Pathway Edits

  • LAR overexpression (leucoanthocyanidin reductase)
  • ANR upregulation (anthocyanidin reductase)
  • DFR promoter enhancement
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Proanthocyanidin branch flux redirectionVacuolar tannin transport optimizationMYB5 transcription factor activationAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
230
IFI-F
—

Use Case

Soft-tannin wine analogs, mild astringency beverages, tea-forward functional drinks, gentle mouthfeel modulation in RTD products. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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