IFI-A · Tannin Grade

Specimen SS-F-AST-032 // ASTRINGENCY · IFI-A

Tannin Grade IV-IV-B

Vitis vinifera

Grape

Tier IVTannin Grade

Target Trait

359.38 mg/L

mg/L

Condensed tannin accumulation

Technical Specifications

Genomic Edits

8

GC Content

41.7%

Construct Length

5,180 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

179.69

Pathway Edits

  • LAR overexpression (leucoanthocyanidin reductase)
  • ANR upregulation (anthocyanidin reductase)
  • DFR promoter enhancement
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Proanthocyanidin branch flux redirectionVacuolar tannin transport optimizationMYB5 transcription factor activationSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
179.69
IFI-F
—

Use Case

Soft-tannin wine analogs, mild astringency beverages, tea-forward functional drinks, gentle mouthfeel modulation in RTD products. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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