IFI-A · Tannin Grade

Specimen SS-F-AST-022 // ASTRINGENCY · IFI-A

Tannin Grade III-PR-B

Vitis vinifera

Grape

Tier IIITannin Grade

Target Trait

258.75 mg/L

mg/L

Condensed tannin accumulation

Technical Specifications

Genomic Edits

7

GC Content

44.1%

Construct Length

4,680 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

129.38

Pathway Edits

  • LAR overexpression (leucoanthocyanidin reductase)
  • ANR upregulation (anthocyanidin reductase)
  • DFR promoter enhancement
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Proanthocyanidin branch flux redirectionVacuolar tannin transport optimizationMYB5 transcription factor activationPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
129.38
IFI-F
—

Use Case

Soft-tannin wine analogs, mild astringency beverages, tea-forward functional drinks, gentle mouthfeel modulation in RTD products. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$199 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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