IFI-A · Tannin Grade

Specimen SS-F-AST-012 // ASTRINGENCY · IFI-A

Tannin Grade II-BL-B

Vitis vinifera

Grape

Tier IITannin Grade

Target Trait

158.13 mg/L

mg/L

Condensed tannin accumulation

Technical Specifications

Genomic Edits

5

GC Content

43.8%

Construct Length

3,860 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

79.06

Pathway Edits

  • LAR overexpression (leucoanthocyanidin reductase)
  • ANR upregulation (anthocyanidin reductase)
  • DFR promoter enhancement
  • Biolistic tungsten-particle coating for leaf/seed explant delivery
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Proanthocyanidin branch flux redirectionVacuolar tannin transport optimizationMYB5 transcription factor activationHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
79.06
IFI-F
—

Use Case

Soft-tannin wine analogs, mild astringency beverages, tea-forward functional drinks, gentle mouthfeel modulation in RTD products. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$149 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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