ATCC-4124 Y10-FULL-FD

Saccharomyces cerevisiae

Yeast

ATCC-4124 SeriesTier 10

Target Trait

1500

mg/L

Reported target: 1500 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

24,200 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

ATCC-4124

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
Full 9-gene cassette: pAMT, Pun1, BCAT, KAS, ACPS, AT3, CYP76AB1, POR, HCT
Four-locus genomic integration (HO, URA3, LEU2, HIS3)

Key Interventions

Complete 9-gene S. cerevisiae capsaicin pathwayCodon-optimized for yeast (~41% GC)Balanced flux via constitutive array

Application Domains

Food ManufacturingIndustrial

Intended Use

Robust extreme-condition chassis producing 1500 mg/L capsaicin under stress fermentation; appropriate for food-grade fermentation and industrial capsaicin production..

Access

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