ATCC-4124 Y8-PAL+-FD

Saccharomyces cerevisiae

Yeast

ATCC-4124 SeriesTier 8

Target Trait

800

mg/L

Reported target: 800 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

25,600 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

ATCC-4124

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
PAL1 overexpression (GAL1 inducible)
Enhanced phenylalanine ammonia-lyase flux

Key Interventions

PAL1 overexpression drives phenylpropanoid branchElevated cinnamic acid intermediate for capsaicin

Application Domains

Food ManufacturingIndustrial

Intended Use

Robust extreme-condition chassis producing 800 mg/L capsaicin under stress fermentation; appropriate for food-grade fermentation and industrial capsaicin production..

Access

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