ATCC-4124 Y2-FAST-FD

Saccharomyces cerevisiae

Yeast

ATCC-4124 SeriesTier 2

Target Trait

100

mg/L

Reported target: 100 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

28,600 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

ATCC-4124

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
ADH1 fast-onset promoter swap for pAMT/Pun1
RNR1 repressor deletion for early S-phase expression

Key Interventions

ADH1 promoter drives early capsaicin onsetFast-onset configuration minimizes lag phase

Application Domains

Food ManufacturingIndustrial

Intended Use

Robust extreme-condition chassis producing 100 mg/L capsaicin under stress fermentation; appropriate for food-grade fermentation and industrial capsaicin production. with fast-onset expression profile.

Access

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