ATCC-4124 Y1-POX--FD

Saccharomyces cerevisiae

Yeast

ATCC-4124 SeriesTier 1

Target Trait

mg/L

Reported target: 50 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

20,900 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

ATCC-4124

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
ΔPOX1 (fatty acid beta-oxidation knockout)
Full 9-gene cassette + POX deletion

Key Interventions

POX1 knockout increases acyl-CoA poolImproved vanillylamine acylation efficiency

Application Domains

Food ManufacturingIndustrial

Intended Use

Robust extreme-condition chassis producing 50 mg/L capsaicin under stress fermentation; appropriate for food-grade fermentation and industrial capsaicin production..

Access

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