Commercial Access Only

Specimen SS-COM-YST-0762 // YEAST

PE-2 Y9-BASE-PH

Saccharomyces cerevisiae

Yeast

PE-2 SeriesTier 9

Target Trait

1000

mg/L

Reported target: 1000 mg/L capsaicin

Technical Specifications

Genomic Edits

9

GC Content

(41%)

Construct Length

22,500 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

PE-2

Pathway Edits

  • ARO4(K229L) feedback-resistant
  • ARO7(G141S) feedback-resistant
  • ΔARO10 (phenylpyruvate decarboxylase knockout)
  • ΔPAD1 (phenylacrylic acid decarboxylase knockout)
  • pAMT genomic integration (TEF1 promoter)
  • Pun1 integration (PGK1 promoter)

Key Interventions

Minimal capsaicin branch (pAMT + Pun1 + BCAT)Feedback-resistant chorismate pathway

Application Domains

PharmaceuticalResearch

Intended Use

High-yield Brazilian industrial strain achieving up to 1000 mg/L capsaicin; optimized for pharmaceutical-grade capsaicin APIs and research applications..

Access

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