PE-2 Y4-QUAD-FD

Saccharomyces cerevisiae

Yeast

PE-2 SeriesTier 4

Target Trait

200

mg/L

Reported target: 200 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

26,000 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

PE-2

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
Pun1(S39L+L345G) dual-mutant variant
Dual ESM2 variant integration

Key Interventions

Pun1 dual-mutant expands substrate scopeDual ESM2 variant improves pathway throughput

Application Domains

Food ManufacturingIndustrial

Intended Use

High-yield Brazilian industrial strain achieving up to 200 mg/L capsaicin; optimized for food-grade fermentation and industrial capsaicin production. with dual-mutant Pun1 expanded-scope variant.

Access

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